qScript™ cDNA Synthesis Kit contains qScript™ Reverse Transcriptase, a mixture of a MMLV reverse transcriptase derivative and ribonuclease inhibitor protein, optimised for sensitive and reliable cDNA synthesis over a broad dynamic range of input RNA. cDNA synthesis reagents are provided in a variety of SuperMix and kit formats to suit specific applications.
Supermix format ideal for high throughput applications
Unbiased representation gives confidence that RNA is represented accurately in the resulting cDNA
Broad dynamic range ensures data from precious samples is more reliably attained
Choice of priming methods to suit individual needs
qScript™ cDNA SuperMix is an easy to use solution for 2-step RT-PCR. This one-tube supermix contains all components for cDNA synthesis including buffer, dNTPs, MgCl₂, primers, RNase inhibitor, qScript™ Reverse Transcriptase and stabilisers. Only RNA needs to be added. Complete cDNA synthesis is achieved in 40 minutes. Consistency and reproducibility are achieved over a broad dynamic range regardless of RNA input. In addition, qScript™ cDNA SuperMix offers excellent sensitivity and accurate representation of low abundance genes. The supermix format eliminates multiple component additions providing exceptional reproducibility and precision. qScript™ cDNA SuperMix is available in both single tube or 96-well format.
qScript™ cDNA Synthesis Kit is a sensitive and easy to use kit for RNA quantification using two-step RT-PCR. This kit contains an optimised blend of random and oligo (dT) primers for robust, consistent and unbiased first-strand synthesis over a broad range of RNA template concentrations. The qScript™ Reaction Mix (5X) contains all required reagents for cDNA synthesis, except for qScript™ Reverse Transcriptase and RNA. qScript™ Reverse Transcriptase is provided in a separate tube. This kit is intended for use by researchers using Bio-Rad iScript™ cDNA Synthesis Kit for ongoing studies or established Standard Operating Procedures that cannot readily be switched to qScript™ cDNA SuperMix.
qScript™ Flex cDNA Kit is an easy to use format for first-strand cDNA synthesis that supports multiple RNA priming strategies. The qScript™ Flex reaction mix (5X) is optimised for use with oligo dT, random or gene specific primers in any combination. A proprietary cDNA-priming enhancer that improves sensitivity is provided pre-mixed with random and oligo dT primers, and in a separate tube for use with gene specific primers (user provided). qScript™ Reverse Transcriptase is provided in a separate tube.
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