AccuPOL DNA Polymerase is a thermostable enzyme that possesses 3 to 5' exonuclease proofreading ability, which enables the polymerase to correct nucleotide misincorporation errors. AccuPOL is recommended for applications which require extremely high fidelity with low error rate. PCR fragments generated with AccuPOL DNA Polymerase are also ideal for blunt end cloning.
- The choice for high fidelity amplifications
- Provides 16× higher fidelity than Taq DNA polymerase
- Optimal for blunt end cloning
- Processes up to 3 kb with extremely high fidelity
Optimal reaction conditions are achieved by using the 10X Key Buffer containing 15 mM MgCl₂ provided with the enzyme. A separate vial of 25 mM MgCl₂ is also included in case a higher MgCl₂ concentration is required for a specific reaction.
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