PerfeCTa® qPCR SuperMixes are 2X concentrated, ready to use reaction cocktails containing all components except primers, probe and template for qPCR.
- Broad dynamic range for greater reliability
- Superior antibody mediated hot start enables higher specificity leading to more accurate quantification
These SuperMixes include buffers and stabilisers optimised for maximum PCR efficiency, reproducibility and sensitivity with TaqMan® and TaqMan® MGB probe chemistries. The enhanced specificity of PerfeCTa® qPCR SuperMixes suppresses cross reactivity between homologous sequences, improving detection and discrimination in SNP applications. A key component is AccuStart™ Taq DNA polymerase that contains monoclonal antibodies, which bind and inactivate the polymerase prior to the initial PCR denaturation step. Upon heat inactivation (2 minutes at 95 °C) the antibodies irreversibly denature releasing fully active, unmodified Taq DNA polymerase. This promotes specific and efficient primer extension with the convenience of room temperature reaction assembly. Perfecta® qPCR SuperMixes are formulated with and without reference dye to provide compatibility with specific Real-Time qPCR instruments.
Sample kits are available, please enquire.