PerfeCTa® SYBR® Green SuperMixes and FastMixes™ enable efficient, sensitive and precise quantitative PCR with proprietary buffers and SYBR® Green stabilisers that maximise fluorescent signal, PCR efficiency, and reduce primer dimers. These SuperMixes and FastMixes™ have been optimised for all Real-Time PCR instrument platforms, including those requiring normalisation with ROX reference dye or fluorescein. UNG versions include uracil DNA glycosylase.
- FastMixes™ offer shorter run times enabling more experiments per day
- Broad dynamic range ensures data from precious samples is more reliably attained
- Superior antibody-mediated hot start results in higher specificity leading to more accurate quantification
- Fast Cycling using existing primer sets means no re-optimisation and increased productivity
PerfeCTa® SYBR® Green SuperMixes are 2X concentrated, ready to use reaction cocktails containing all components for qPCR, except primers and template. A key component of these SuperMixes is AccuStart™ Taq DNA polymerase, which enables specific and efficient primer extension with the convenience of room temperature reaction assembly. AccuStart™ Taq DNA polymerase contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation the antibodies denature irreversibly, releasing fully active and unmodified Taq DNA polymerase.
PerfeCTa® SYBR® Green FastMixes™ are 2X concentrated, ready to use reaction mixes delivering maximum PCR efficiency, sensitivity, specificity and robust fluorescent signal using either fast or conventional cycling protocols. Rapid cycling is achieved by instant activation of AccuStart™ Taq DNA polymerase coupled with rapid polymerisation kinetics. High performance qPCR can be achieved in as little as 33 minutes.