PerfeCTa® qPCR FastMix® II is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. PerfeCTa® qPCR FastMix® II is provided as a 2X concentrated ready to use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue colour of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimise pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.
A key component of PerfeCTa® qPCR FastMix® II is an ultra-pure, processive thermostable DNA polymerase that is free of detectable E. coli DNA. PerfeCTa® qPCR FastMix® II is ideal for demanding qPCR applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy samples. The enzyme in PerfeCTa® qPCR FastMix® II is combined with high avidity monoclonal antibodies to provide a stringent automatic Hot Start that allows reaction assembly, and temporary storage, at room temperature prior to PCR amplification.
Delivery information: Kits are available with or without ROX reference dye. All kits comprise of 2X reaction buffer containing optimised concentrations of MgCl₂, dNTPs (dATP, dCTP, dGTP, dTTP), Hot Start DNA polymerase, AccuVue™ blue qPCR dye, and stabilisers.